치과용 지르코니아의 in vitro 및 in vivo 생물학적 평가
In vitro and in vivo biocompatibility evaluation of dental zirconia ceramic
최유리, 김경남, 김광만,
소속 상세정보
최유리 ( Choi Yu-Ri ) - 연세대학교 치과대학 치과생체재료공학교실
김경남 ( Kim Kyoung-Nam ) - 연세대학교 치과대학 치과생체재료공학교실
김광만 ( Kim Kwang-Man ) - 연세대학교 치과대학 치과생체재료공학교실
KMID : 1035320150420010065
Abstract
Recently, zirconia ceramics have been extensively used as the artificial tooth in modern dentistry on account of their excellent mechanical properties and good esthetics. The purpose of this study was to evaluate biocompatibility of zirconia ceramics using agar diffusion test, acute systemic toxicity, intracutaneous test and local lymph node assay(LLNA). For in vitro test, the agar diffusion assay was used sintered zirconia specimens (In-ceram YZ-55, VITA Co., Bad sackingen, Germany) were performed according to ISO 7405. For in vivo test, three different tests were carried out and samples were prepared according to ISO 10993-12 for all of the test. Acute systemic toxicity test and Intracutaneous test, were performed according to ISO 10993-11 and ISO 10993-10. The murine local lymph node assay test was performed according to OECD guide line 442 B. The overall criterion for the cytotoxicity of zirconia sample was "0". For the acute systemic toxicity test, no symptoms were observed during the visual observation of the animal. For the intracutaneous irritation test, there was no redness and swellings on the reaction sites. The result of LLNA test, the samples extractions in polar solvents showed average OD value of 1.078 and those in non-polar solvents showed average OD value of 1.059. The partially stabilized zirconia materials were proved to be biocompatible. The results of both in vitro and in vivo test concluded that the zirconia materials were biocompatible.
키워드
Ziroconia; Biocompatibility
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